We previously demonstrated that cholesterol deprivation increases endothelial cyclooxygenase-2 (COX-2)-dependent prostacyclin [prostaglandin I2 (PGI2)] production in vitro. Cholesterol directly regulates gene transcription through the sterol response element binding protein (SREBP). In this work. we demonstrate that SREBP directly regulates COX-2 expression. https://www.danddcollectiblers.shop/product-category/cholesterol-synthesis/